Fed-batch enzymatic hydrolysis of steam-exploded sugarcane bagasse

Statistical design and mathematical modeling were used to investigate the fed-batch enzymatic hydrolysis of steam-exploded sugarcane bagasse (195 °C, 7.5 min). First, a Box-Behnken experimental was evaluate effect enzyme loading (8, 24, 40 FPU g-1 glucans Cellic CTec3®), stirring speed (100, 150, 200 rpm), substrate total solids (5, 12.5, 20 wt%) on release glucose equivalents (GlcEq, mostly glucose) after for 48 h in batch mode. A simplified kinetic model fit data, which specific activities CTec3 not differentiated, adsorption ignored, end-product inhibition only attributed accumulation. The adjusted predict effects intermittent feedings experiments. Compared with experiments at wt%, proposed procedure able increase GlcEq productivity by nearly 68% using same loading, producing hydrolysates containing 91.8 g L-1 GlcEq.